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human high sensitivity t-cell discovery array 14-plex (hdhstc14  (Eve Technologies Corporation)

 
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    Eve Technologies Corporation human high sensitivity t-cell discovery array 14-plex (hdhstc14
    Human High Sensitivity T Cell Discovery Array 14 Plex (Hdhstc14, supplied by Eve Technologies Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+high+sensitivity+t-cell+discovery+array+14-plex/human+high+sensitivity+t+cell+discovery+array+14+plex++hdhstc14/nct05806385-145-18-10
    Average 90 stars, based on 1 article reviews
    human high sensitivity t-cell discovery array 14-plex (hdhstc14 - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: Transcutaneous auricular vagus nerve stimulation (taVNS) for the treatment of pediatric nephrotic syndrome: a pilot study
    Article Snippet: Cytokines were quantified using the Human High Sensitivity T-Cell Discovery Array 14-Plex (Eve Technologies Corp, Calgary, AB, Canada).



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    Schematic representation of commonly used immunoassays and colour-development protocols in sputum research: (A) AP-based ELISA, (B) HRP-based ELISA, It is to be noted that some ELISA protocols like the R&D system duo-sets, follows a peroxidase based colour development for biotinylated secondary antibodies. An intermediate streptavidin-HRP complex reagent is used where the streptavidin binds covalently to the biotin while the HRP catalyzes the substrate to generate the chromogenic product. (C) <t>Milliplex</t> or bead-based multiplex system employs polystyrene beads surface-conjugated with capture antibodies. Essentially, the sandwich structure (capture antibody-antigen-detection antibody) of an ELISA is maintained but without the requirement of an enzymatic reaction. Instead, the detection is based on Streptavidin-conjugated phycoerythrin (PE, emission at 578 nm) which covalently binds to the biotinylated detection antibody. (D) IL-8 absolute values obtained from n=47 samples analysed by the three methods, Data represented as median (Interquartile range). Kruskal-Wallis test, Dunn multiple comparison test. P<0.05 considered as significant difference.
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    Schematic representation of commonly used immunoassays and colour-development protocols in sputum research: (A) AP-based ELISA, (B) HRP-based ELISA, It is to be noted that some ELISA protocols like the R&D system duo-sets, follows a peroxidase based colour development for biotinylated secondary antibodies. An intermediate streptavidin-HRP complex reagent is used where the streptavidin binds covalently to the biotin while the HRP catalyzes the substrate to generate the chromogenic product. (C) Milliplex or bead-based multiplex system employs polystyrene beads surface-conjugated with capture antibodies. Essentially, the sandwich structure (capture antibody-antigen-detection antibody) of an ELISA is maintained but without the requirement of an enzymatic reaction. Instead, the detection is based on Streptavidin-conjugated phycoerythrin (PE, emission at 578 nm) which covalently binds to the biotinylated detection antibody. (D) IL-8 absolute values obtained from n=47 samples analysed by the three methods, Data represented as median (Interquartile range). Kruskal-Wallis test, Dunn multiple comparison test. P<0.05 considered as significant difference.

    Journal: Journal of immunological methods

    Article Title: Endogenous peroxidases in sputum interfere with horse-radish peroxidase-based ELISAs

    doi: 10.1016/j.jim.2017.11.005

    Figure Lengend Snippet: Schematic representation of commonly used immunoassays and colour-development protocols in sputum research: (A) AP-based ELISA, (B) HRP-based ELISA, It is to be noted that some ELISA protocols like the R&D system duo-sets, follows a peroxidase based colour development for biotinylated secondary antibodies. An intermediate streptavidin-HRP complex reagent is used where the streptavidin binds covalently to the biotin while the HRP catalyzes the substrate to generate the chromogenic product. (C) Milliplex or bead-based multiplex system employs polystyrene beads surface-conjugated with capture antibodies. Essentially, the sandwich structure (capture antibody-antigen-detection antibody) of an ELISA is maintained but without the requirement of an enzymatic reaction. Instead, the detection is based on Streptavidin-conjugated phycoerythrin (PE, emission at 578 nm) which covalently binds to the biotinylated detection antibody. (D) IL-8 absolute values obtained from n=47 samples analysed by the three methods, Data represented as median (Interquartile range). Kruskal-Wallis test, Dunn multiple comparison test. P<0.05 considered as significant difference.

    Article Snippet: IL-8 levels were hence analysed with a Millipore Milliplex kit (Human High Sensitivity T-Cell Discovery Array 14-plex) on BioPlex 200 system by Eve Technologies Inc., (Calgary, Alberta, Canada).

    Techniques: Enzyme-linked Immunosorbent Assay, Multiplex Assay

    Correlation of IL-8 data set generated by (A) AP-based ELISA (B) HRP-based ELISA against Bead-based Milliplex system (n = 47); Values of IL-8 sputum levels measured by (C) AP-ELISA and (D) HRP-ELISA plotted against presence of sputum EPX, Pearson. P<0.05 is considered to be significant.

    Journal: Journal of immunological methods

    Article Title: Endogenous peroxidases in sputum interfere with horse-radish peroxidase-based ELISAs

    doi: 10.1016/j.jim.2017.11.005

    Figure Lengend Snippet: Correlation of IL-8 data set generated by (A) AP-based ELISA (B) HRP-based ELISA against Bead-based Milliplex system (n = 47); Values of IL-8 sputum levels measured by (C) AP-ELISA and (D) HRP-ELISA plotted against presence of sputum EPX, Pearson. P<0.05 is considered to be significant.

    Article Snippet: IL-8 levels were hence analysed with a Millipore Milliplex kit (Human High Sensitivity T-Cell Discovery Array 14-plex) on BioPlex 200 system by Eve Technologies Inc., (Calgary, Alberta, Canada).

    Techniques: Generated, Enzyme-linked Immunosorbent Assay